Intermediate

Part:BBa_K191001:Design

Designed by: Le Thanh Tu NGUYEN   Group: iGEM09_EPF-Lausanne   (2009-10-11)

Promoter LacI - RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • LacI promoter was cut with E and S
  • Plasmid with RBS was cut with E and X
  • LacI fragment is then ligated into the RBS plasmid.


Source

BBa_R0010 and BBa_B0030

References